Depletion of p21-activated kinase 1 up-regulates the immune system of APCΔ14/ mice and inhibits intestinal tumorigenesis

N Huynh; K Wang; M Yim; CJ Dumesny; MS Sandrin; GS Baldwin; M Nikfarjam; H He

Journal article

Depletion of p21-activated kinase 1 up-regulates the immune system of APCΔ14/ mice and inhibits intestinal tumorigenesis

N Huynh, K Wang, M Yim, CJ Dumesny, MS Sandrin, GS Baldwin, M Nikfarjam, H He

BMC Cancer | BIOMED CENTRAL LTD | Published : 2017

DOI: 10.1186/s12885-017-3432-0

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Abstract

Background: P21-activated kinase 1 (PAK1) stimulates growth and metastasis of colorectal cancer (CRC) through activation of multiple signalling pathways. Up-regulation of CRC stem cell markers by PAK1 also contributes to the resistance of CRC to 5-fluorouracil. The aim of this study was to investigate the effect of PAK1 depletion and inhibition on the immune system and on intestinal tumour formation in APCΔ14/+ mice. Methods: The PAK1 KO APCΔ14/+ mice were generated by cross-breeding of PAK1 KO mice with APCΔ14/+ mice. Splenic lymphocytes were analysed by flow cytometry, and immunohistochemical staining. The numbers of intestinal tumours were counted. Blood cells were also counted. Results: ..

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University of Melbourne Researchers

Mauro Sandrin Author

Mehrdad Nikfarjam Author

Hong He Author

Related Projects (1)

Targetting the Kinase PAK1 in Colorectal Cancer

This project aims to develop a new therapy for colorectal cancer (CRC). We have already demonstrated that a molecule called PAK1 is the mast..

Grants

Awarded by Austin Medical Research Foundation

Funding Acknowledgements

This work was supported by grants from the National Health and Medical Research Council of Australia (1,041,831, GSB/AS; 1,020,983, GSB), and the Austin Medical Research Foundation (HH).